rrmch-central-research-lab

Central Research Lab

Overview

Advances in medicine and science come from new ideas and approaches developed through research. Central Research Laboratory (CRL) is a facility that provides centralized research infrastructure to benefit investigators in multiple disciplines across RajaRajeswari Medical College and Hospital (RRMCH). CRL is Located on the second floor of the college building and our mission is to provide a comfortable, safe and effective environment to conduct professional, high-quality research. This is achieved by providing the necessary environment, equipment and personnel to achieve research goals. CRL acts as a single point of contact for investigators to accelerate their research study and provides structured environment where researchers, undergraduates, Postgraduates and clinicians can work together on projects that are relevant and fundamentally important. Genomics Lab at CRL is useful for doing research at the advanced level as well as for diagnosis and confirmation of certain genetic disorders at the molecular level. This facility is extensively used by our staff for completing their External and Internal Research funding projects, postgraduates for completing their thesis/research projects as well as by undergraduates to complete their STS ICMR projects and for any short term research projects.

Why Choose Us?

The CRL has dedicated and well qualified faculty, infrastructure and state of art research facilities for carrying out advanced research. The Raja RajaRajeswari Medical college supports all aspects of biomedical research, from exploration at the basic science level to translating fundamental discoveries into treatments that can improve human health and cure certain ailments. This has made us a leading institution with a focus on both traditional and interdisciplinary research. Also, it extends into fostering the development of the next generation of exceptional research scholars and scientists currently studying undergraduate and post graduate medical course.

With the addition of the Molecular Biology and Molecular Genetics programs, RajaRajeswari medical college and hospital now integrally involved in both basic and clinical research, and is among the relatively few institutions with a consistent and concerted focus on bridging basic and clinical science. With an eye toward designing specific treatments and cures for disease, the Laboratory’s research has the potential to affect us all.

  Department will be geared towards promotion and co-ordination of basic, applied and clinical research including clinical trials and operational research in areas related to medical, health, biomedical and medical profession, and education. This shall be achieved through development of infrastructure, manpower and skills in cutting edge areas. It will be accomplished by supporting infrastructure development and Extramural funded programs.

Facilities

R & D Facilities available for Research

The following facilities are available in the Central Research Lab, Rajarajeswari Medical College & Hospital for carrying out advanced research.

Molecular Biology:
PCR and RT PCR for research and diagnosis.
Proteomics work and Biochemical Assays:

HPLC: Quantification of antibiotics in Blood Serum, Quantification of Vitamins in Blood serum and Identification and Quantification of New Medicinal components.

TLC: Identification of Biological Components.

Nano Drop Spectrophotometer: Quantification of proteins and nucleic acids. LAF: Mammalian cell culture
Fully automated IHC/ISH slide staining instrument (BenchMark XT): For IHC, ISH, and FITC tests and to know status of estrogen receptor (ER), progesterone receptor (PR) in Breast cancer patients.

Cobas e 411 analyzer: The measuring cell uses an amplified signal to detect ultra-low concentrations of analyte. By precisely controlling electrically-initiated reactions, Electrochemiluminescence(ECL) technology virtually eliminates unnecessary repeats and reruns, providing accurate results the first time.

Thyroid Function Anti-Tg Anti-TPO Anti TSH-receptor FT3 FT4 T3 T4 T-Uptake Tg TSH Fertility / Hormones ACTH C-peptide Cortisol DHEA-S Estradiol free ¢-HCG FSH HCG+¢ HCG STAT Insulin LH PAPP-A Progesterone Prolactin SHBG Testosterone Anemia Ferritin Vitamin B12 Folate RBC Folate
Cardiac CK-MB (mass) CK-MB (mass) STAT Digoxin Digitoxin Myoglobin Myoglobin STAT NT-proBNP Troponin T Troponin T STAT Troponin I* Troponin T HS* Maternal Care AFP free ß HCG hCG+ ß PAPP-A PLGF sFLt Tumor Markers AFP CA 125 II CA 15-3 CA 19-9 CA 72-4 CEA CYFRA 21-1 free PSA NSE S-100 total PSA
Critical Care IL6 PCT S- 100 Infectious Disease Anti-HAV Anti-HAV IgM Anti-HBc Anti-HBc IgM Anti-Hbe Anti-HBs Anti-HCV HBsAg confirmatory CMV IgG CMV IgM HBeAg HBsAg HIV Antigen HIV Antigen confirmatory HIV combi Rubella IgG Rubella IgM Toxo IgG Toxo IgM Bone Markers ß-CrossLaps 25-(OH)2 Vitamin D3 Intact PTH N-MID Osteocalcin PTH STAT Total-P1NP
Others IgE S-100 (brain damage) anti-CCP

Animal Cell culturing: CO2 Incubator
Microscopy: Fluorescent Microscope for diagnosis and Research.
Clinical Neurophysiology: EEG and ENMG, Autonomic Nervous System Testing, Pulmonary Function Testing System

Equipments for Research Activities:

1.High-performance Liquid Chrmatography ( HPLC; Shimadzu; LC2010CHT; Japan) 2.Nanodrop Spectrophotometer (ND-2000C; Thermo, USA) 3.UV/Visible Spectrophotometer (Chemline, India) 4. Gel documentation system (ProteinSimple, USA) 5.Gradient PCR (C1000, Biorad;USA) 6.Gradient PCR (Simpli Amp, ABI;USA) 7.Vertical Electrophoresis unit (Biorad;USA) 8.Horizontal Electrophoresis Unit (Biorad;USA) 9.Horizontal Electrophoresis Unit (Mupid-eXU;Japan) 10.CO2 Incubator (Thermo, USA) 11.Centrifuges- refrigerated (5804; Eppendorf; Germany) 12.Fluorescent Microscope (Olympus- BX 43; Japan) 13.RT PCR (ABI- Step one;USA) 14.Digital Colorimeter (Scientex, India) 15.Refrigerators (0 to 8oC) 16.Freezer (- 20oC; Elanpro; Itlay ) 17.Deep Freezer (- 40oC;Biocare;India ) 18.Deep Freezer (- 80oC; Eppendorf; Germany) 19.Cobas e 411 (Roche-Hitachi;USA) Autoanalyzer 20.Fully automated IHC/ISH slide staining instrument (BenchMark GX instrument;USA) 21.Electronic balances (Sartorius;BSA 224S-CW; Germany) 22.Ice making Machine (Elanpro; Itlay) 23.PH Meter (Sartorius;PP50; Germany) 24.Laminar Air Flow (Fortunex;India) 25.K.F. Titrator (LASCO;India) 26.Rotavap (Superfit; India) 27.BOD Incubator 28.Digital Clinical Flame photometer 29.Binocular microscopes (Olympus) 30.Computerized Treadmill System 31.EEG- Electroencephalogram Neuropack S1; Nihon Khoden; Japan 32.ENMG- Electroneuromyogram- Nihon Khoden; Japan 33.Spirowin (Pulmonary Function Testing System; LifeLine Meditech, India 34.Canwin (Autonomic Nervous Testing Equipment; LifeLine Meditech, India) 35.S T win Deluxe Version 6.1 (Nassan) (Stress Testing Machine) 36.Revela 12 (12 channel digital Holter) version 6.2 (Nassan) 37.Microtome 38.Paraffin embedding bath 39.Tissue floatation waterbath 40.Helena Electrophoresis Unit with power pack for Hemoglobin electrophoresis 41.Water plant (Elega;UK) 42.Hot air oven (Sentwin, India) 43.Bacteriological Incubator (Apollo Scientific, India) 44.Autoclaves 45.Water bath

Faculty

Research

Collaborative Research

1.Institute of Wood Science, Govt. of India, Bangalore.


Sl. No

Project Title

Principal Investigators

Co- Investigators

Departments

1

To Study Hypoglycemic, Anti-inflammatory and Antioxidant Properties of Momordica Cymbalaria and Garcinia indica Extracts on Streptozotocin- Induced Diabetic Rats.

Dr.S. Prabhakara

Chandrasekhar B.S

Dr. Basavaraj

Bhandare

Mr. Mohan T

Dr. Anabazhagan Kolandaswamy

Central Research Lab,

Chemistry of Forest Products Division,

Pharmacology.

2

Cardioprotective Activity of Rubia cordifolia in Albino rats

Chandrasekhar B.S

Dr.S. Prabhakara

Dr. Basavaraj

Bhandare

Mr. Mohan T

Dr. Anabazhagan Kolandaswamy

Chemistry of Forest Products Division

Pharmacology

Genetics



RajaRajeswari Dental College and Hospital, Bangalore.

Sl. No

Project Title

Principal Investigators

Co- Investigators

Departments

1

To evaluate and compare cytotoxicity of various endodontic irrigants on human cultured periodontal ligament cells: An invitro study.

Dr. Apoorva Saxena

Dr.S. Prabhakara

Mr. Mohan T

Dental surgery and Central Research Lab

2

Comparative evaluation of antimicrobial activity of propolis, Neem leaves, Green Tea, Sodium Hypochlorite and Saline as root canal irrigants by Microbial culturing and quantification in chronically exposed anterior primary health.

Dr. Stiya Ann Varghese

Dr.S. Prabhakara

Mr. Mohan T

Pedodontics& Preventive Dentistry and Central Research Lab

3

Evaluation of the pH change and calcium ion release of calcium hydroxide with different vehicles: An invitro study.

Dr. A Sadiya Naaz

Dr.S. Prabhakara

Mr. Mohan T

Dental surgery and Central Research Lab



External Funding Research Projects

Sl. No

Project Title

Funding from

Principal Investigator

Co investigators

Departments

1.

Genetic investigations in patients suffering from paroxysmal dyskinesias and Benign familial infantile epilepsy

VGST, Karnataka Govt.

Dr. S. Prabhakara,

Dr. Prasad B.N.K,

Dr.Kishore K.K,

Dr. E. Adarsh,

Dr. K . Anbazhagan,

Central Research Lab,

Neurology, Pediatrics.

2.

A Comparative evaluation of effects of newer antiepileptic drugs (Leviriracetam/topiramate) on bone health using Biological markers

ICMR,

Govt. of India

Dr. Chanda Kulkarni

-

Pharmacology

Central Research Lab

3.

Screening for early prediction and diagnosis of pre-eclampsia in indian women by congo red dot test and by quantifying amyloid proteins in pre-eclampsia patients

RGUHS, Govt. Of Karnataka

Dr. M.P.A. Sailakshmi

Dr. S. Prabhakara

Dr. Anbazhagan K

OBG

Central Research Lab

4.

“Quantification of circulating microRNAs associated with post-menopausal osteoporosis”

RGUHS Govt. Of Karnataka

Dr. B.M. Rupakala

Dr. Anbazhagan. K

OBG

Central Research Lab

5.

GADA & ICA positivity in

Population: need for early

insulinization to prevent β cell

Destruction

RGUHS Govt. Of Karnataka

Dr. Priyadarshini

Dr. Deepti Gupta

Biochemistry

6.

Utilization pattern and

Effectiveness of cognitive

Behavior therapy for anxiety

Disorders in adults: a

Naturalistic study

RGUHS Govt. Of Karnataka

Dr. Sudhir Hebbar

_

Psychiatry



Ongoing Research Projects

Sl. No

Title

Principal Investigator

Co Investigators

Department

1.

Early diagnosis of Preeclampsia in Indian Women using Congo Red Dot test.

Dr. M.P.A Sai Lakshmi

Dr.S.Prabhakara

Dr. Anabazhagan.k

OBG

Central Research lab

2.

Quantification of circulating microRNAs associated with post-menopausal osteoporosis”

Dr. B.M. Rupakala

Dr. Anbazhagan. K

OBG

Central Research Lab

3.

To Study Hypoglycemic, Anti-inflammatory and Antioxidant Properties of Momordica Cymbalaria and Garcinia indica Extracts on Streptozotocin- Induced Diabetic Rats.

Dr.S. Prabhakara

Chandrasekhar B.S Dr. Basavaraj Bhandare

Dr. Anabazhagan Kolandaswamy

Mr. Mohan T

Central Research Lab

Chemistry of Forest Products Division

Pharmacology

Molecular Biology

4.

Extent and pattern of substance abuse and pharmacotherapeutic profile among patients attending a tertiary care hospital

Dr. Chanda Kulkarni

Dr. Sahajanand

Dr. Meena KN

Dr. Amar Bavle

Pharmacology

Psychiatry

Anesthesia

Central Research Lab

5.

Genetic investigations in patients suffering from paroxysmal dyskinesias and Benign familial infantile epilepsy

Dr. S. Prabhakara

Dr. Prasad B.N.K

Dr. Kishore K.K

Dr. E. Adarsh

Dr. K. Anbazhagan

Central Research Lab

Neurology

Pediatrics

6.

microRNAs in Development of Osteoclasts During Post-Menopausal Osteoporosis in Indian Women

Dr. Anabazhagan Kolandaswamy

Dr. S. Prabhakara

Dr. Roshankumar

Dr. Sailakshmi

Dr. H.V. Shetty

Central Research Lab

Orthopedics

OBG

Biochemistry

7.

Phytotherapeutic characterization and exploration of UV – photoactivated Tridax procumbens Linn for antibacterial activity against multi-drug resistant (MDR) bacteria and anti-inflammatory activity.

Dr. R C Kanta

Dr. Basavaraj

Bhandare

Mr. Mohan T

Microbiology

Pharmacology

Central Research Lab

8.

Hormonal and hormonal receptor profile in children with defective

Androgenization

Dr. Girish M L

Dr. Manjunath.Y.A

Dr. S. Prabhakara

Dr. Anabazhagan k

Pediatrics Surgery

Pathology

Central Research lab

9.

Interleukin-6 and Serum Cortisol levels in Septic shock syndrome

Dr. Krishna MV

-

General Medicine

10.

To Evaluate and compare cytotoxicity of various endodontic irrigants on human cultured periodontal ligament cells: An invitro study.

Dr. Apoorva Saxena

Dr.S. Prabhakara

Mr. Mohan T

Dental surgery and Central Research Lab

11.

Postoperative cognitive dysfunction after general anesthesia in elderly-comparison of sevoflurane with desflurane in day care surgeries and identification of genetic biomarkers

Dr. Vani S

Dr. Rangalakshmi

Dr. Sahajanand .H

Anesthesia

12.

Phytotherapeutic Beneficiary Effects of Ashwagandha (Withania somnifera) in postmenopausal women with hypothyroidism and vitamin-D deficiency

Dr. Mamtha B Patil

Dr. Sahajanand .H

Dr. Basavaraj

Bhandare

Mr. Mohan T

General Medicine

Anesthesia

Pharmacology

Central Research Lab

13.

Comparative evaluation of antimicrobial activity of propolis, Neem leaves, Green Tea, Sodium Hypochlorite and Saline as root canal irrigants by Microbial culturing and quantification in chronically exposed anterior primary health.

Dr. Stiya Ann Varghese

Dr.S. Prabhakara

Mr. Mohan T

Pedodontics& Preventive Dentistry and Central Research Lab

14.

GADA & ICA positivity in

Population: need for early

insulinization to prevent β cell

Destruction

Dr. Priyadarshini

Dr. Deepti Gupta

Biochemistry

15.

Maternal serum 25-hydroxy vitamin by HPLC and measures of newborn and placental weight

Dr. Meena

Dr. Sailaxmi Dr. H.Sahajanda Dr. Prabhakara. S

Mr. Mohan. T

Pharmacology, Anesthesia,

OBG

CRL

16.

An open labeled, cross sectional study to compare blood pralidoxime levels and clinical outcome in patients treated with continuous pralidoxime infusion versus intermittent dosing in Organophosphorous poisoning using RP-HPLC

Dr. Basavaraj

Bhandare

Dr. Pavithra K Dr.Satyanarayana Dr. Krishna MV Dr.Prabhakara.S

Mr. Mohan. T

Pharmacology

General Medicine

CRL

17.

Evaluation of the pH change and calcium ion release of calcium hydroxide with different vehicles: An invitro study.

Dr. A Sadiya Naaz

Dr.S. Prabhakara

Mr. Mohan T

Dental surgery and Central Research Lab

18.

Study of lipoprotein (a) levels in hypothyroidism

Dr. H.V. Shetty

-

Biochemistry

19.

A Study of triglyceride/HDL ratio as a marker of insulin resistance in healthy young adults

Dr. S.M.R. Usha

-

Biochemistry

20.

Study of lipid profile in smokers and non-smokers

Dr. Priyadarshini

-

Biochemistry

21.

Study of serum homocysteine levels in vitiligo patients

Dr. C.M. Bindu

-

Biochemistry

22.

Study of cystatin C levels in Type 2 diabetics without complications

Dr. Deepti Gupta

-

Biochemistry

23.

Impact of Laproscopic Ovarian Drilling on anti mullerian hormone levels in polycystic ovarian syndrome prior to ovarian drilling and post ovarian drilling

Dr. B.M. Rupakala

Dr. A. G. Shruthi

OBG

24.

A case control study to correlate relation between material serum homocysteine levels and IUGR

Dr. B.M. Rupakala

Dr. N. Amitha

OBG

News

Awards

Dr. S. Prabhakara:
Young Scientist award from Karnataka Government for the year 2014-2015.

Dr. Anbazhagan K:
Ramalingaswami Fellowship, 2016 from DBT, Govt. of India. Senior Research Fellowship, 2008 from CSIR, Govt. of India.

Publications

1.Prabhu M.R, Sahajanand H, Dwajani.S. Diagnostic use of bone biomarkers and drugs affecting bone remodelling. Indian Journal of Pharmacy and Pharmacology 2016; 3(4) ; 155-161

2.Mohan T, Prabhakara S, Anbazhagan K, Pavithra  Krishnan, Basavaraj Bhandare, Sahajanand H. Development and Validation of new RP-HPLC Method for Quantitative  Estimation of Vitamin-D3 in Bulk drug and Pharmaceutical Dosage Form. Indian Drugs 2016; 53(6); 40-45.

3.Karamaradi S Nirmala, Bhat Manjula S, Sahajanand H. Effect of Two types of dietary ghee on serum lipid levels in rats. J Evolution Med Dent 2016; 5(49); 3240-3244.

4.Chanda Kulkarni, GRK.Sarma, Dwajani.S, Errol.F, Sheela.CN. Teratogenicity of women with epilepsy on antiepileptic drugs: a prospective and retrospective observational study Neurology Asia 2015; 2(6); 127-129.

5.Mohan T, Prabhakara S , Pavithra Krishnan , Basavaraj Bhandare, Sahajanand H. A New method development and validation for estimation of paracetamol in pharmaceutical dosage form by rp-hplc. Int j pharm pahrm sci 2015;7 (8): 190-194.

6.Dwajani.S, GRK.Sarma, Ganapathy Bantwal, Chanda Kulkarni. Effects of Levetiracetam and Topiramate on bone health in adults, using bone specific biomarkers Medico Research Chronicles 2015; 2(1):61-66

7.Sahajanda H, Sudheer Rao. Effects of intravenously administered Clonidine on Haemodynamics and on Plasma Cortisol level during Laparoscopic Cholecystectomies" Indian Journal of Anaesthesia 2015;59(1):53-55.

8.Prabhakara S, Anbazhagan Kolandaswamy. Molecular analysis of PRRT2 gene in a case of Paroxysmal kinesiegenic dyskinesia patient. Ann Indian Acad of Neurol 2014; 17:459-62 (Corresponding Author).

9.Dwajani.S, Ranjana.G, GRK.Sarma, Chanda Kulkarni. Influence of Antiepileptic Drugs on Haematological and Biochemical parameters in Patients with Epilepsy. International Journal of Basic and Clinical Pharmacology 2014; 3(4):692-95. 

10.Ranjana.G, Dwajani.S, GRK.Sarma, Chanda Kulkarni. The sociodemographic, clinical and pharmacotherapy characteristics influencing quality of life in patietns with epilepsy – A cross sectional study Journal of neurosciences in rural practice 2014; 5 suppl 1:23-28.

11.Chikkanna Venkatashamappa, Prabhakara S, Sahajanda H. A New method for Simultaneous estimation of ampicillin sodium and sulbactam sodium in injectable dosage form by reverse phase high performance liquid chromatography IJSID 2014; 4 (1):71-78.

12.Prashanth Kumar M, Karamaradi S Nirmala, Shobana Sahajanand, Sahajanda H, Ranganath MD, Sathyamurthy B, Mohamed Nizam Al Deen Shah. Visual evoked potentials in subjects performing dual tasks Journal of Medical Sciences 2013; 31-34.

13.Prabhakara S. Need for Molecular Genetic Investigation on patients suffering from paroxysmal dyskinesias Journal of Medical Sciences 2013; 7-11.

Paper Presentation

Curriculum

Will be updated soon................

Faculty

  • Dr. Sahajananda H

    Chief Coordinator
  • Dr. S. Prabhakara

    Senior Research Scientist & Associate Coordinator
  • Dr. Chanda Kulkarni

    Research Consultant & Professor
  • Dr. Anbazhagan Kolandaswamy

    Visiting Research Consultant
  • Dr. Dwajani. S

    Senior Research Associate
  • Dr. K.S. Nirmala

    Neurophysiology Technologist
  • Mr. Basanth Kumar Reddy

    Research Scientist
  • Mr. Mohan.T

    In charge of Instrumentation
  • Mr. Prabhu.S

    Research Assistant
  • Mr. Nepolian S

    Clinical Research Coordinator
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